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fluorescent tagged mouse igg 1 , igg 2a , and igg 3 detection kit  (Cellular Technology Ltd)


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    Cellular Technology Ltd fluorescent tagged mouse igg 1 , igg 2a , and igg 3 detection kit
    Dorsal root ganglion (DRG) neurons release antigen-specific IgG 1 . (A) Representative images from total IgG ELISpot assay performed on DRG neurons isolated from alum-injected and alum + KLH-immunized mice. Quantitation of total IgG-releasing DRG neurons isolated from (B) alum-injected and alum + KLH-immunized mice ( n = 5 per group) or (C) alum-injected and alum + ovalbumin (OVA)-immunized mice ( n = 5 per group) using the ELISpot assay (mean ± SD); ** P < 0.01 vs . alum-injected (Mann-Whiteny test). (D) Quantitation of anti-OVA-releasing DRG neurons isolated from alum-injected and alum + OVA-immunized mice ( n = 5 per group) using the Anti-OVA IgG ELISpot assay (mean ± SD); ** P < 0.01 vs. alum-injected (Mann-Whitney test). (E) Quantification of IgG 1 -, IgG <t>2a</t> -, and IgG 3 -specific FluoroSpots from DRG neurons isolated from alum-injected and alum + KLH-immunized mice using the IgG isotype FluoroSpot assay; (mean ± SD, n = 4 per group); * P < 0.05 vs . alum-injected (Mann-Whitney test). (F) Quantification of anti-OVA- and anti-KLH-specific IgG immunospots from DRG neurons isolated from alum-injected and alum + KLH-immunized mice (mean ± SD, n = 4 per group) using the antigen-specific ELISpot assay; * P < 0.05 vs . alum-injected for each antigen (Mann-Whitney test).
    Fluorescent Tagged Mouse Igg 1 , Igg 2a , And Igg 3 Detection Kit, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/igg+3+detection+kit/pmc05932385-132-11-24?v=Cellular+Technology+Ltd
    Average 90 stars, based on 1 article reviews
    fluorescent tagged mouse igg 1 , igg 2a , and igg 3 detection kit - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Immunization Elicits Antigen-Specific Antibody Sequestration in Dorsal Root Ganglia Sensory Neurons"

    Article Title: Immunization Elicits Antigen-Specific Antibody Sequestration in Dorsal Root Ganglia Sensory Neurons

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2018.00638

    Dorsal root ganglion (DRG) neurons release antigen-specific IgG 1 . (A) Representative images from total IgG ELISpot assay performed on DRG neurons isolated from alum-injected and alum + KLH-immunized mice. Quantitation of total IgG-releasing DRG neurons isolated from (B) alum-injected and alum + KLH-immunized mice ( n = 5 per group) or (C) alum-injected and alum + ovalbumin (OVA)-immunized mice ( n = 5 per group) using the ELISpot assay (mean ± SD); ** P < 0.01 vs . alum-injected (Mann-Whiteny test). (D) Quantitation of anti-OVA-releasing DRG neurons isolated from alum-injected and alum + OVA-immunized mice ( n = 5 per group) using the Anti-OVA IgG ELISpot assay (mean ± SD); ** P < 0.01 vs. alum-injected (Mann-Whitney test). (E) Quantification of IgG 1 -, IgG 2a -, and IgG 3 -specific FluoroSpots from DRG neurons isolated from alum-injected and alum + KLH-immunized mice using the IgG isotype FluoroSpot assay; (mean ± SD, n = 4 per group); * P < 0.05 vs . alum-injected (Mann-Whitney test). (F) Quantification of anti-OVA- and anti-KLH-specific IgG immunospots from DRG neurons isolated from alum-injected and alum + KLH-immunized mice (mean ± SD, n = 4 per group) using the antigen-specific ELISpot assay; * P < 0.05 vs . alum-injected for each antigen (Mann-Whitney test).
    Figure Legend Snippet: Dorsal root ganglion (DRG) neurons release antigen-specific IgG 1 . (A) Representative images from total IgG ELISpot assay performed on DRG neurons isolated from alum-injected and alum + KLH-immunized mice. Quantitation of total IgG-releasing DRG neurons isolated from (B) alum-injected and alum + KLH-immunized mice ( n = 5 per group) or (C) alum-injected and alum + ovalbumin (OVA)-immunized mice ( n = 5 per group) using the ELISpot assay (mean ± SD); ** P < 0.01 vs . alum-injected (Mann-Whiteny test). (D) Quantitation of anti-OVA-releasing DRG neurons isolated from alum-injected and alum + OVA-immunized mice ( n = 5 per group) using the Anti-OVA IgG ELISpot assay (mean ± SD); ** P < 0.01 vs. alum-injected (Mann-Whitney test). (E) Quantification of IgG 1 -, IgG 2a -, and IgG 3 -specific FluoroSpots from DRG neurons isolated from alum-injected and alum + KLH-immunized mice using the IgG isotype FluoroSpot assay; (mean ± SD, n = 4 per group); * P < 0.05 vs . alum-injected (Mann-Whitney test). (F) Quantification of anti-OVA- and anti-KLH-specific IgG immunospots from DRG neurons isolated from alum-injected and alum + KLH-immunized mice (mean ± SD, n = 4 per group) using the antigen-specific ELISpot assay; * P < 0.05 vs . alum-injected for each antigen (Mann-Whitney test).

    Techniques Used: Enzyme-linked Immunospot, Isolation, Injection, Quantitation Assay, MANN-WHITNEY, Flurospot



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    Dorsal root ganglion (DRG) neurons release antigen-specific IgG 1 . (A) Representative images from total IgG ELISpot assay performed on DRG neurons isolated from alum-injected and alum + KLH-immunized mice. Quantitation of total IgG-releasing DRG neurons isolated from (B) alum-injected and alum + KLH-immunized mice ( n = 5 per group) or (C) alum-injected and alum + ovalbumin (OVA)-immunized mice ( n = 5 per group) using the ELISpot assay (mean ± SD); ** P < 0.01 vs . alum-injected (Mann-Whiteny test). (D) Quantitation of anti-OVA-releasing DRG neurons isolated from alum-injected and alum + OVA-immunized mice ( n = 5 per group) using the Anti-OVA IgG ELISpot assay (mean ± SD); ** P < 0.01 vs. alum-injected (Mann-Whitney test). (E) Quantification of IgG 1 -, IgG <t>2a</t> -, and IgG 3 -specific FluoroSpots from DRG neurons isolated from alum-injected and alum + KLH-immunized mice using the IgG isotype FluoroSpot assay; (mean ± SD, n = 4 per group); * P < 0.05 vs . alum-injected (Mann-Whitney test). (F) Quantification of anti-OVA- and anti-KLH-specific IgG immunospots from DRG neurons isolated from alum-injected and alum + KLH-immunized mice (mean ± SD, n = 4 per group) using the antigen-specific ELISpot assay; * P < 0.05 vs . alum-injected for each antigen (Mann-Whitney test).
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    igg 3 detection kit  (Cellular Technology Ltd)
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    Dorsal root ganglion (DRG) neurons release antigen-specific IgG 1 . (A) Representative images from total IgG ELISpot assay performed on DRG neurons isolated from alum-injected and alum + KLH-immunized mice. Quantitation of total IgG-releasing DRG neurons isolated from (B) alum-injected and alum + KLH-immunized mice ( n = 5 per group) or (C) alum-injected and alum + ovalbumin (OVA)-immunized mice ( n = 5 per group) using the ELISpot assay (mean ± SD); ** P < 0.01 vs . alum-injected (Mann-Whiteny test). (D) Quantitation of anti-OVA-releasing DRG neurons isolated from alum-injected and alum + OVA-immunized mice ( n = 5 per group) using the Anti-OVA IgG ELISpot assay (mean ± SD); ** P < 0.01 vs. alum-injected (Mann-Whitney test). (E) Quantification of IgG 1 -, IgG <t>2a</t> -, and IgG 3 -specific FluoroSpots from DRG neurons isolated from alum-injected and alum + KLH-immunized mice using the IgG isotype FluoroSpot assay; (mean ± SD, n = 4 per group); * P < 0.05 vs . alum-injected (Mann-Whitney test). (F) Quantification of anti-OVA- and anti-KLH-specific IgG immunospots from DRG neurons isolated from alum-injected and alum + KLH-immunized mice (mean ± SD, n = 4 per group) using the antigen-specific ELISpot assay; * P < 0.05 vs . alum-injected for each antigen (Mann-Whitney test).
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    Image Search Results


    Dorsal root ganglion (DRG) neurons release antigen-specific IgG 1 . (A) Representative images from total IgG ELISpot assay performed on DRG neurons isolated from alum-injected and alum + KLH-immunized mice. Quantitation of total IgG-releasing DRG neurons isolated from (B) alum-injected and alum + KLH-immunized mice ( n = 5 per group) or (C) alum-injected and alum + ovalbumin (OVA)-immunized mice ( n = 5 per group) using the ELISpot assay (mean ± SD); ** P < 0.01 vs . alum-injected (Mann-Whiteny test). (D) Quantitation of anti-OVA-releasing DRG neurons isolated from alum-injected and alum + OVA-immunized mice ( n = 5 per group) using the Anti-OVA IgG ELISpot assay (mean ± SD); ** P < 0.01 vs. alum-injected (Mann-Whitney test). (E) Quantification of IgG 1 -, IgG 2a -, and IgG 3 -specific FluoroSpots from DRG neurons isolated from alum-injected and alum + KLH-immunized mice using the IgG isotype FluoroSpot assay; (mean ± SD, n = 4 per group); * P < 0.05 vs . alum-injected (Mann-Whitney test). (F) Quantification of anti-OVA- and anti-KLH-specific IgG immunospots from DRG neurons isolated from alum-injected and alum + KLH-immunized mice (mean ± SD, n = 4 per group) using the antigen-specific ELISpot assay; * P < 0.05 vs . alum-injected for each antigen (Mann-Whitney test).

    Journal: Frontiers in Immunology

    Article Title: Immunization Elicits Antigen-Specific Antibody Sequestration in Dorsal Root Ganglia Sensory Neurons

    doi: 10.3389/fimmu.2018.00638

    Figure Lengend Snippet: Dorsal root ganglion (DRG) neurons release antigen-specific IgG 1 . (A) Representative images from total IgG ELISpot assay performed on DRG neurons isolated from alum-injected and alum + KLH-immunized mice. Quantitation of total IgG-releasing DRG neurons isolated from (B) alum-injected and alum + KLH-immunized mice ( n = 5 per group) or (C) alum-injected and alum + ovalbumin (OVA)-immunized mice ( n = 5 per group) using the ELISpot assay (mean ± SD); ** P < 0.01 vs . alum-injected (Mann-Whiteny test). (D) Quantitation of anti-OVA-releasing DRG neurons isolated from alum-injected and alum + OVA-immunized mice ( n = 5 per group) using the Anti-OVA IgG ELISpot assay (mean ± SD); ** P < 0.01 vs. alum-injected (Mann-Whitney test). (E) Quantification of IgG 1 -, IgG 2a -, and IgG 3 -specific FluoroSpots from DRG neurons isolated from alum-injected and alum + KLH-immunized mice using the IgG isotype FluoroSpot assay; (mean ± SD, n = 4 per group); * P < 0.05 vs . alum-injected (Mann-Whitney test). (F) Quantification of anti-OVA- and anti-KLH-specific IgG immunospots from DRG neurons isolated from alum-injected and alum + KLH-immunized mice (mean ± SD, n = 4 per group) using the antigen-specific ELISpot assay; * P < 0.05 vs . alum-injected for each antigen (Mann-Whitney test).

    Article Snippet: FluoroSpots were developed using the fluorescent tagged mouse IgG 1 , IgG 2a , and IgG 3 detection kit as per the manufacturer’s protocol (Cellular Technology Ltd.) and scanned and analyzed at Cellular Technology Ltd.

    Techniques: Enzyme-linked Immunospot, Isolation, Injection, Quantitation Assay, MANN-WHITNEY, Flurospot